ABSTRACT
Background & objectives: Accurate and early diagnosis is imperial in the management of endometriosis, endometrioid carcinoma of ovary (ECO) and endometrioid endometrial cancer (EC), yet there are no definitive diagnostic methods available for these diseases. Therefore, the present study was aimed to evaluate the diagnostic potential of differentially expressed miRNAs in serum samples of women with endometriosis, ECO and EC to establish them as diagnostic biomarkers. Methods: Blood samples (5 ml) were obtained from 40 patients (n=10/study group) undergoing laparoscopy/laparotomy/hysterectomy. miRNA-rich RNA was extracted from the serum samples, and quantitative real-time (qRT)-PCR was performed to check the expression levels of miR-16, miR-99b, miR-20a, miR-145, miR-143 and miR-125a in all the samples. Receiver operating characteristic (ROC) curve analysis was done to check the diagnostic potential. Results: In endometriosis, miR-16 was downregulated (P<0.05) whereas miR-99b, miR-125a, miR-143 and miR-145 were upregulated (P<0.05). In ECO group, downregulated expression of miR-16 and miR-125a (P<0.05) was observed, whereas miR-99b, miR-143 and miR-145 were upregulated (P<0.05). In endometrioid EC, miR-16, miR-99b, miR-125 and miR-145 were downregulated (P<0.05), whereas miR-143 was upregulated (P<0.05). ROC curve analysis showed that, for endometriosis, miR-99b, miR-125a, miR-143 and miR-145 served as diagnostic markers. miR-145 showed diagnostic power for ECO, and for endometrioid EC, miR-16, miR-99b, miR-125a and miR-145 showed diagnostic potential. Interpretation & conclusions: The present findings suggested that certain circulating miRNAs (miB99b, miR-16, miR-125a, miR-145) might act as indicators and discriminators of endometriosis and endometrioid subtypes of EC and ovarian cancer and might serve as potential biomarkers for early diagnosis and management of these debilitating diseases.
ABSTRACT
Objectives: The efficacy of single oral dose KetorolacTromethamine, Tramadol and Placebo was evaluated in paincontrol after periodontal surgery.Materials and Methods: The study design is a split mouthstudy involving three quadrants of the same patient. 20patients requiring flap surgeries in at least three quadrantswere recorded. Patients were randomly divided into threegroups as per the medications given, either 10 mg ketorolac or50 mg tramadol or placebo tablets at least 30 minutes beforeadministration of local anesthesia (LA). The duration of surgeryfrom the time of incision to the placement of the last suture isrecorded. After the completion of the surgery, patients wereasked to rate their subjective operative pain intensity using avisual analog scale.Results: Differences were statistically significant in VAS scorebetween ketorolac and placebo, and tramadol and placebogroup. Comparison of sum of pain intensity showedsignificantly greater pain levels in the placebo than in theketorolac group and tramadol group.Conclusion: The results of this single-dose, parallel-group,and double blind placebo-controlled study showed that 10-mgketorolac and 50-mg tramadol administered immediately beforeperiodontal surgery was effective for better response by thepatient during the procedure. However, ketorolac and tramadolpremedication neither affected delayed pain levels, norpostoperative analgesic consumption.
ABSTRACT
Objective: Periodontal disease results the production ofdifferent enzymes that are released by epithelial orinflammatory cells. There are important enzymes associatedwith cellular alteration and cell death like: Lactatedehydrogenase (LDH), Creatine Kinase (CK), Alkaline andAcidic phosphatase (ALP, ACP). Enzymatic activity changesresults in changes health and metabolic changes in Gingivaland Periodontal condition.Design of study: We have examined the activity of CK, LDH,ALP and ACP in saliva from patients with periodontal diseasebefore and after periodontal treatment (experimental group –40samples) and in saliva from healthy patients (control group –30 samples). Periodontal disease was determined based onclinical parameters (gingival index (GI), bleeding on probing(BOP), probing depth (PD). Patients with periodontal diseasewere under periodontal treatment.Results: Results showed statistically significant increases ofactivity of CK, LDH, ALP and ACP in saliva from patients withperiodontal disease in relation to control group. There ispositive correlation between the examined salivary enzymesand value of the gingival index. After periodontal therapy theactivity of all salivary enzymes was significantly decreased.Conclusions: Based on these results, it can be stated thatactivity of these enzymes in saliva, as biochemical markers forperiodontal tissue damage, may be useful in diagnosis,prognosis and evaluation of therapy effects in periodontaldisease.
ABSTRACT
Background: Due to low sensitivity and inability to detect drug resistance, smear microscopy limits its impact on TB control. Culture methods and drug susceptibility testing is complex, time consuming, and takes around 6-8 weeks. A new diagnostic test, cartridge based nucleic acid amplification test (CBNAAT) was developed based on real-time polymerase chain reaction (RT PCR). Objective of this study to compare the results of CBNAAT for diagnosis of pulmonary tuberculosis with LED fluorescent microscopy and sputum culture.Methods: A cross-sectional study was conducted in the department of Chest and TB, CIMS, Bilaspur. Each Sputum sample of presumptive TB patients were tested with CBNAAT, sputum smear microscopy by light emitting diode (LED) fluorescent microscopy (FM) and solid and liquid culture for diagnosis of Tuberculosis. Results of CBNAAT, Fluorescent Microscopy and Culture for detection of Mycobacterium Tuberculosis were compared.Results: The sensitivity and specificity for CBNAAT were 97% and 100% respectively; while that for Fluorescent microscopy were 70% and 100% respectively. The positive and negative predictive value for CBNAAT was 100% and 96% respectively. The positive and negative predictive value for Fluorescent microscopy was 100% and 73% respectively.Conclusion: CBNAAT is having high sensitivity and specificity for diagnosis of pulmonary tuberculosis. It should be routinely used under national health programme to detect a tuberculosis case efficiently.